图书简介
This updTed edTion colleTs cTting-edgeTechniques usedTo Tudy neural Tem and progenTor cells as well asThe brain microenvironmeT. FeTuring a wide range ofTechnological advances inThe Tudy of neural Tem cells,The volume highligTsThe promises of Tem cell-basedTherapeTic applicTions for ceTral nervous syTem ailmeTs. WrTten forThe highly successful MThods in Molecular Biology series, chaTers include iTroduTionsToTheir respeTiveTopics, liTs ofThe necessary mTerials and reageTs, Tep-by-Tep, readily reproducible laborTory prTocols, andTips onTroubleshoTing and avoiding known pTfalls.AThorTTive and praTical, Neural ProgenTor Cells: MThods and PrTocols, Second EdTion serves as an invaluable resource forThe neT generTion of neuroscieTiTs asThey develop innovTive experimeTal paradigms and progressTowardTherapeTic applicTions inThe field of neurobiology.
Culturing Mouse Fetal Neural PrCursorCells in a Free-Floating Serum-FreeCondition.- Isolation andCulture of Neural Stem/ProgenitorCells from the Postnatal PeriventrCular Region.- Isolation andCulture of Adult HippCampal PrCursorCells as Free-Floating Neurospheres.- Isolate andCulture Neural StemCells from the Mouse Adult SpinalCord.-Culturing and Expansion of ClinCal Grade” Neural StemCells from the Fetal HumanCentral Nervous System.- MCroglia Isolation from Neural StemCell-EnrChed Regions.- MCroglial FCS Sorting for Robust RNA RCovery for Next Generation SequeCing.- Method for Isolating ExtrCellular VesCles from Human Neural StemCells Expanded under NeurosphereCulture.- Isolating andCulturing of PrCursorCells from the Adult Human Brain.- Isolation andCulture of PrCursorCells from the Adult Human SpinalCord.- Isolation and EnrChment of Defined NeuralCell Populations from Heterogeneous Neural StemCell Progeny.- Identifying Neural ProgenitorCells in the Adult Human Brain.- Neonatal Transplant in HypoxC Injury.- Isolation and PurifCation of Self Renewable Human Neural StemCells from iPCs forCell Therapy in Experimental Model of IChemC Stroke.- GeneratingCerebral Organoids from Human Pluripotent StemCells.- AdvaCing Our Understanding of Brain Disorders: ReseaCh Using Postmortem Brain Tissue.- Biovalue in Human Brain Banking: ApplCations andChallenges for ReseaCh in NeurodegenerativeDiseases.
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