图书简介
Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods.
However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since.
This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy.
This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods.
The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine.
Key features:
The first collective reference to collate all known knowled
Part I Basic principles of tissue optical clearing Chapter 1. Tissue optical clearing mechanisms Tingting Yu, Dan Zhu, Luis Oliveira, Elina A. Genina, Alexey N. Bashkatov, Valery V. Tuchin Chapter 2. Tissue optical clearing for Mueller matrix microscopy Nan Zeng, Honghui He, Valery V. Tuchin, and Hui Ma Chapter 3. Traditional and innovative optical clearing agents Elina Genina, Vadim D. Genin, Jingtan Zhu, Alexey Bashkatov, Dan Zhu, Valery Tuchin Chapter 4. Chemical enhancers for improving tissue optical clearing efficacy Dan Zhu, Yanmei Liang, Xingde Li, and Valery Tuchin Chapter 5. Human skin autofluorescence and optical clearing Walter Blondel, Marine Amouroux, Sergey Zaytsev, Elina Genina, Victor Colas, Christian Daul, Alexander Pravdin and Valery Tuchin Chapter 6. Molecular modeling of post-diffusion phase of optical clearing of biological tissues K.V. Berezin, K. N. Dvoretskiy, M. L. Chernavina, M.A. Likhter, and V.V. Tuchin Chapter 7. Refractive index measurements of tissue and blood components and OCAs in a wide spectral range Ekaterina N. Lazareva, Luis Oliveira, Irina Yu. Yanina, Nikita V. Chernomyrdin, Guzel R. Musina, Daria K. Tuchina, Alexey N. Bashkatov, Kirill I. Zaytsev, and Valery V. Tuchin Chapter 8. Water migration at skin optical clearing Anton Yu. Sdobnov, Johannes Schleusener, Jurgen Lademann, Valery V. Tuchin, Maxim E. Darvin Chapter 9. Optical and mechanical properties of the cartilage during optical clearing Yulia Alexandrovskaya, Olga Baum, Vladimir Zaitsev, Alexander Sovetsky, Alexander Matveyev, Lev Matveev, Kirill Larin, Emil Sobol, Valery Tuchin Chapter 10. Compression optical clearing Olga Zurukina, Yury P. Sinichkin Part II Tissue optical clearing method for biology (3D imaging) Chapter 11. Optical clearing for multiscale tissues and the quantitative evaluation of clearing methods in mouse organs Tingting Yu, Jianyi Xu, Dan Zhu Chapter 12. Ultrafast aqueous clearing methods for 3D imaging Tingting Yu, Jingtan Zhu, Dan Zhu Chapter 13. The Challenges and Opportunities in Hydrophilic Tissue learing Methods Etsuo A. Susaki, Hiroki R. Ueda Chapter 14. Combination of tissue optical clearing and 3D fluorescence microscopy for high-throughput imaging of entire organs and organisms Peng Fei and Chunyu Fang Chapter 15. Endogenous fluorescence preservation from solvent-based optical clearing Tingting Yu, Yisong Qi, Dan Zhu Chapter 16. Progress in ex situ tissue optical clearing - shifting immuno-oncology to the third dimension Pawel Matryba, Leszek Kaczmarek, Jakub Golab Part III Towards in vivo tissue optical clearing Chapter 17. In vivo skin optical clearing methods for blood flow and cell imaging Dongyu Li, Wei Feng, Rui Shi, Dan Zhu Chapter 18. In vivo skull optical clearing for imaging cortical neuron and vascular structure and function Dongyu Li, Yanjie Zhao, Chao Zhang, Dan Zhu Chapter 19. In vivo skin optical clearing in humans Elina Genina, Alexey N. Bashkatov, Valery Tuchin, Vladimir Zharov Chapter 20. Optical clearing of blood and blood components Olga Zhernovaya, Elina A. Genina, Valery V. Tuchin, Alexey N. Bashkatov Chapter 21. Blood and lymph flow imaging at optical clearing Polina A. Dyachenko (Timoshina), Arkady C. Abdurashitov, Oxana V. Glushkovskaya, and Valery V. Tuchin Part IV Combination of tissue optical clearing and optical imaging/spectroscopy for diagnostics Chapter 22. Optical clearing aided photoacoustic imaging in vivo Yong Zhou and Lihong V. Wang Chapter 23. Enhancement of contrast in photoacoustic - fluorescence tomography and cytometry using optical clearing and contrast agents Julijana Cvjetinovic, Daniil Nozdriukhin, Maksim Mokrousov, Alexander Novikov, Marina Novoselova, Valery Tuchin, Dmitry Gorin Chapter 24. Tissue optical clearing in the terahertz range Olga Smolyanskaya, Kirill Zaytsev, Irina Dolganova, Guzel Musina, Daria Tuchina, Maxim Nazarov, Alexander Shkurinov, Valery Tuchin Chapter 25. Magnetic resonance imaging study of diamagnetic and paramagnetic agents for optical clearing of tumor-specific fluorescent signal in vivo Alexei A. Bogdanov Jr., Natalia I. Kazachkina, Victoria V. Zherdeva, Irina G. Meerovich, Daria K. Tuchina, Ilya D. Solovyev, Alexander P. Savitsky, Valery V. Tuchin Chapter 26. Use of an optical clearing and index matching agents to enhance the imaging of caries lesions and internal structures in teeth using optical coherence tomography and SWIR imaging Daniel Fried Chapter 27. Optical clearing of adipose tissue Irina Yu. Yanina, Yohei Tanikawa, Daria K. Tuchina, Polina A. Dyachenko (Timoshina), Yasunobu Iga, Shinichi Takimoto, Elina A. Genina, Alexey N. Bashkatov, Georgy S. Terentyuk, Nikita A. Navolokin, Alla B. Bucharskaya, Galina N. Maslyakova, Valery V. Tuchin Chapter 28. Diabetes mellitus-induced alterations of tissue optical properties, optical clearing efficiency and molecular diffusivity Daria Tuchina, Valery Tuchin Chapter 29. Tissue optical clearing for in vivo detection and imaging diabetes induced changes in cells, vascular structure and function Dongyu Li, Wei Feng, Rui Shi, Valery Tuchin, Dan Zhu Chapter 30. Light operation on cortex through optical clearing skull window Dongyu Li, Chao Zhang, Oxana Glushkovskaya, Yanjie Zhao, Dan Zhu Chapter 31. The role of optical clearing to enhance the applications of in vivo OCT and photodynamic therapy: towards PDT of pigmented melanomas and beyond? Layla Pires, Michelle Barreto Requena, Valentin Demidov, Ana Gabriela Salvio, I. Alex Vitkin, Brian C. Wilson, Cristina Kurachi Chapter 32. Combination of tissue Optical clearing and OCT for tumor diagnosis via permeability coefficient measurements Qingliang Zhao Chapter 33. Optical clearing for cancer diagnostics and monitoring Luis Oliveiro, Valery Tuchin Chapter 34. Contrast enhancement and tissue differentiation in optical coherence tomography with mechanical compression Pavel Agrba and Mikhail Kirillin Chapter 35. Measurement of the dermal beta-carotene in the context of the multimodal optical clearing M. A. Ansari, V.V. Tuchin Chapter 36. Optical clearing and molecular diffusivity of hard and soft oral tissues Alexey A. Selifonov and Valery V. Tuchin Chapter 37. Optical clearing and Raman spectroscopy: in vivo applications Qingyu Lin, Ekaterina N. Lazareva, Vyacheslav I. Kochubey, Yixiang Duan, and Valery V. Tuchin
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