Cancer Metabolism:Methods and Protocols

Cancer Metabolism:Methods and Protocols（Methods in Molecular Biology）

癌症代谢：方法与协议
肿瘤学

原价：

￥ 3650

售价：

优惠

平台大促低至8折优惠

发货周期：外国库房发货，通常付款后3-5周到货

作者

Majda Haznadar, National Cancer Institute, North Bethesda, MD, USA

出版社

Humana Press

出版时间

2019年02月07日

装帧

精装

ＩＳＢＮ

9781493990269

复制

页码

495

语种

英文

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暂无评分

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图书简介

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories.

Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction.- Imaging Cancer Metabolism with Positron Emission Tomography (PET).- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells.- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics.- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM).- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells.- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment.- Quantitation of Macropinocytosis in Cancer Cells.- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation.- Methods to Measure Autophagy in Cancer Metabolism.- Lipidomic Analysis of Cancer Cell and Tumor Tissue.- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids.- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites.- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy.- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging.- Compositional Analysis of the Human Microbiome in Cancer Research.- Assessing Metabolic Dysregulation in Muscle During Cachexia.- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells.- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging.- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods.- Hyperpolarized MRI for Studying Tumor Metabolism.- Overview of Glutamine-Dependency and Metabolic Rescue Protocols.- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype.- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways.- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism.

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