Neural Progenitor Cells:Methods and Protocols

图书简介

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing “no new neuron” doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.

Culturing Fetal Precursors Cells Using Free-Floating Serum-Free Conditions.- In vitro Expansion of Foetal Neural Progenitors as Adherent Cell Lines.- Isolate and Culture Precursor Cells from the Adult Periventricular Area.- Culturing and Expansion of Precursor Cells from the Adult Hippocampus.- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord.- Culturing and Expansion of “Clinical Grade” Precursors Cells from the Foetal Human Central Nervous System.- Isolating and Culturing of Precursor Cells from the Adult Human Brain.- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord.- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny.- Isolation of Adult Stem Cells from the Human Olfactory Mucosa.- Enumerating Stem Cell Frequency – Neural Colony Forming Cell Assay.- Flow Cytometry of Neural Cells.- Neonatal Transplant in Hypoxic Injury.- Isolation and Purification of Self Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke.- Transplantation of Foetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson’s Disease.- Distribution of Neural Precursor Cells in Rodent Brain.- Identifying Neural Precursor Cells in the Adult Human Brain.- Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple.

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Neural Progenitor Cells:Methods and Protocols（Methods in Molecular Biology） 神经病学

Neural Progenitor Cells:Methods and Protocols（Methods in Molecular Biology）
神经病学