Axon Degeneration

图书简介

This book is a colleTion of classical as well as innovTive mThods usedTo inveTigTe axon degenerTion wTh a paTicular focus on addressingThe common challenges encouTered while performingThese procedures. PaTicular TteTion is devTedToThe Tudy of axon loss in several model organisms, as each poses unique challenges and provides powerful advaTages. WrTten forThe highly successful MThods in Molecular Biology series, chaTers include iTroduTionsToTheir respeTiveTopics, liTs ofThe necessary mTerials, Tep-by-Tep, readily reproducible laborTory prTocols, andTips onTroubleshoTing and avoiding known pTfalls.AThorTTive and praTical, Axon DegenerTion: MThods and PrTocols is an ideal guide for facilTTingThe applicTion and fuTher developmeT ofThese prTocols, which will helpThe scieTific communTyTackle impoTaT queTions regarding axon degenerTion.ChaTers 2, 3, and 20 are available Open Access under a CreTive Commons TtribTion 4.0 ITernTional License via link.springer.com.

Axon Degeneration: WhCh Method toChoose?.- Axon Degeneration Assays in SuperiorCervCal Ganglion ExplantCultures.- MCroinjCtion of SuperiorCervCal Ganglion Neurons for Studying Axon Degeneration.- Assessing Axonal Degeneration in EmbryonC Dorsal Root Ganglion Neurons In Vitro.- Viral TransdCtion of DRG Neurons.- Planning and Analysis of Axon Degeneration Creening Experiments.- A MCrofluidCCulture Platform to Assess Axon Degeneration.- A ChwannCell-NeuronCoCulture System to Study Neuron-Glia InterCtion during Axon Degeneration.- Establishing MyelinatingCoCultures Using Human iPC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination.- Preparation of OrganotypC HippCampal SlCeCultures for the Study ofCNS Disease and Damage.- OrganotypCCulture Assay for NeuromuCular SynaptC Degeneration and FuCtion.- IntrCellular RCordings of PostsynaptC Voltage Responses at the Drosophila NeuromuCular JuCtion.- Ex Vivo Studies of OptC Nerve Axon ElCtrophysiology.- Ex Vivo Analysis of Axonal Degeneration Using CiatC and OptC Nerve Preparations.- Measuring BioenergetC Signatures of Peripheral Nerve Segments by ExtrCellular Flux Analysis.- DetCtion of Neutrophils in the CiatC Nerve Following Peripheral Nerve Injury.- Whole-Tissue Immunolabeling and 3D FluoreCeCe Imaging to Visualize Axon Degeneration in the IntCt, UnsCtioned Mouse Tissues.- Transmission ElCtron MCroCopy and Morphometry of theCNS White Matter.- In VivoCaCium Imaging During Axon Degeneration in Zebrafish.- In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves.- In Vivo Visualization of Moving SynaptCCargoComplexes within Drosophila Larval Segmental Axons.- AChemotherapy-IndCed Peripheral Neuropathy Model in Drosophila melanogaster.-Models of Axon Degeneration in Drosophila Larvae.- In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster.

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Axon Degeneration（Methods in Molecular Biology (Hardcover)） 轴突退化：方法与协议 神经生物学

Axon Degeneration（Methods in Molecular Biology (Hardcover)）

轴突退化：方法与协议
神经生物学