The green revolution led to the development of improved varieties of crops, especially cereals, and since then, classical or molecular breeding has resulted in the creation of economically valuable species. Thanks to recent developments in biotechnology, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. This technology has made the scientific community aware of the critical role of transgenic, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. Focuses on important field crops to highlight germplasm enhancement for developing resistance to newly emerging diseases, pests, nutrient- and water-use efficiency, root traits and improved tolerance to increasing temperature. Introduces significant recent achievements in crop improvement using methods such as micropropagation, somaclonal variation, somatic embryogenesis, anther/pollen/embryo culture, and compressing the breeding cycle for accelerated breeding and early release of crop varieties. Plant biotechnology has now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenic now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Biotechnology and Crop Improvement: Tissue Culture and Tra

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