图书简介
This detailed collection focuses on current methodologies for studying various crop diseases. The book explores phytopathogen detection, host-pathogen interactions at the earliest stages of colonization, RNA-seq analyses, as well as host responses downstream of, or coincidental to, gene expression and a newly established protocol for gene editing of Medicago sativa. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant-Pathogen Interactions serves as an ideal guide for researchers working in the vital area of plant diseases.
Specific Detection and Quantification of Major Fusarium spp. Associated with Cereal and Pulse Crops.- Distinguishing Puccinia striiformis f. sp. tritici Isolates Using Genomic Sequencing: A Case Study.- DNA-Barcoding Identification of Plant Pathogens for Disease Diagnostics.- Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa in Field.- Selective Quantification of Chemotropic Responses of Fusarium graminearum.- Live Cell Visualization of Early Stages of Root Colonization by the Vascular Wilt Pathogen Fusarium oxysporum.- Transfection of Barley Leaf Protoplasts with a Fluorescently-Tagged Fungal Effector for In Planta Localization Studies in Barley.- A Bioinformatic Guide to Identify Protein Effectors from Phytopathogens.- Unraveling Plant-Pathogen Interactions in Cereals Using RNA-Seq.- RNA-Seq Data Processing in Plant-Pathogen Interaction System: A Case Study.- Differential Expression Feature Extraction (DEFE): A Case Study in Wheat FHB RNA-Seq Data Analysis.- Proteomic Profiling of Host Response in the Cereal Crop Triticum aestivum to the Mycotoxin, 15-Acetyldeoxynivalenol, Produced by the Fungal Pathogen, Fusarium graminearum.- Quantitative Phosphoproteome Analysis of the Interaction between Fusarium graminearum and Triticum aestivum.- Fatty Acid Profiling of Grapevine Extracellular Compartment.- Identifying Fungal Secondary Metabolites and Their Role in Plant Pathogenesis.- Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Improvement of Disease Resistance.
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