图书简介

CCN Proteins - Cellular Communication Network Factors: Expanding their Repertoire toward a New Concept.- Optimal CCN4 Immunofluorescence for Tissue Microarray.- Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes.- Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-free Fractions, and Cells.- Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes.- Evaluation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance.- Protocols for Screening Peptides Binding to CCN Family Proteins and their Extended Utility.- Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism.- Analyses of the Post-Transcriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression.- Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network.- Utilizing Public Molecular Biological Databases for CCN Family Research.- Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation.- Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy.- The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues.- Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis.- Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins.- Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells.- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo.- Angiogenesis Assays for the Analysis of CCN Proteins.- Using the Bleomycin-Induced Model of Fibrosis to Study the Contribution of CCN Proteins to Scleroderma Fibrosis.- Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1.- Immunohistochemical Analysis of CCN2 in Experimental Fracture Healing Models.- Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins.- Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products.- Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain and Loss of Function Approaches.- Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney.- Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis.

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