图书简介
This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry.
Protein Purification Technologies.- High Throughput Process Development: I. Process Chromatography.- 3. High Throughput Process Development: II. Membrane Chromatography.- Media Selection in Ion Exchange Chromatography in a Single Microplate.- High Throughput Screening of Dye-Ligands for Chromatography.- Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers.- Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts.- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins.- Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments.- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview.- Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins.- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery.- An Orthogonal Fusion Tag for Efficient Protein Purification.- Biomimetic Affinity Ligands for Protein Purification.- Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification.- Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening.- ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification.- Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing.- Sample Displacement Batch Chromatography of Proteins.- Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3.- Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins.- Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H .- Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin.- Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry.- Protein Structure Analysis and Validation with X-Ray Crystallography.- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes.- Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.- G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification.- Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐