图书简介

Vaccine Delivery and Immune Response Basics.-Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.-Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.-Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.-Antigen Discovery in Bacterial Pan-Proteomes.-Alphavirus-Based Antigen Preparation.-Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.-Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.-High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.-Production of Zika Virus Virus-Like Particles.-Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).-Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.-Whole-Cell Vaccine Preparation: Options and Perspectives.-O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein.-Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.-Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.-Attenuation Methods for Live Vaccines.-Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.-Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.-Lipofection-Based Delivery of DNA Vaccines.-Flavivirus DNA Vaccine Design and Adjuvant Selection.-Vaccine Delivery with a Detoxified Bacterial Toxin.-Needle-Less or Non-Invasive Delivery Technology.-Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.-A Hybrid Biological-Biomaterial Vector for Antigen Delivery.-Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.-Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.-The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.-Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.-The Quantification of Antigen-Specific T Cells by IFN-? ELISpot.-The Measurement of Antigen-Specific IgG Titers by Direct ELISA.-Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.-Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.-Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.

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