图书简介

Microinjection and Micromanipulation: A Historical Perspective.- Production of Transgenic Mice by Pronuclear Microinjection.- Transgene Recombineering in Bacterial Artificial Chromosomes.- Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice.- Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.- Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer.- Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection.- Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice.- Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique.- Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation.- Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides.- Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection.- The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications.- The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm.- Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line.- Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology.- Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos.- Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells.- Generation of Rabbit Models by Gene Editing Nucleases.- Production of Genetically Engineered Porcine Embryos by Handmade Cloning.- Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production.- Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer.- Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting.- Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes.- Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing.- Microinjection in Zebrafish for Genome Editing and Functional Studies.- Microinjection of Marine Fish Eggs.- Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method.- Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus.- Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function.

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