Avian and Reptilian Developmental Biology:Methods and Protocols

图书简介

This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a variety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

Some Thoughts on Experimental Design.-燙omparative Genomics as a Foundation for Evo-Devo Studies in Birds.-燗 Step-by-Step Guide to Assemble a Reptilian Genome.-燝enomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes.-燬ystems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses using the Chicken Skin Paradigm.-燗pplication of a CAGE Method to an Avian Development Study.-燙RISPR/Cas9 in the Chicken Embryo.-燜luorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development.-燣entiviral-Mediated Transgenesis in Songbirds.-營n Ovo Electroporation Methods in Chick Embryos.-燝enetic Manipulation of the Avian Urogenital System using In Ovo Electroporation.-燛nhancer Analyses using Chicken Embryo Electroporation.-燭ransgene Introduction into the Chick Limb Bud by Electroporation.- Chicken Induced Pluripotent Stem Cells: Establishment and Characterization.-營solation and Characterization of Chicken Primordial Germ Cells and their Application in Transgenesis.-燞andling of Gametes for In Vitro Insemination in Birds.-營n Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells.-燣ifting the Veil on Reptile Embryology: The Veiled Chameleon (Chamaeleo calyptratus) as a Model System to Study Reptilian Development.-燤odel Clades versus Model Species: Anolis Lizards as an Integrative Model of Anatomical Evolution.-燭he Feather Model for Chemo- and Radiation Therapy-Induced Tissue Damage.-燗n Early Chick Embryo Culture Device for Extended Continuous Observation.-燗 Sensitive and Versatile In Situ Hybridization Protocol for Gene Expression Analysis in Developing Amniote Brains.-燬omitogenesis and Axial Development in Reptiles.-燤icro-CT Imaging on Living Alligator Teeth Reveals Natural Tooth Cycling.

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Avian and Reptilian Developmental Biology:Methods and Protocols（Methods in Molecular Biology） 分子生物学

Avian and Reptilian Developmental Biology:Methods and Protocols（Methods in Molecular Biology）
分子生物学