Preeclampsia:Methods and Protocols

Preeclampsia:Methods and Protocols（Methods in Molecular Biology）
分子生物学

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作者

Padma Murthi, Monash University, Clayton, VIC; Cathy Vaillancourt, INRS-Institut Armand-Frappier, Laval, QC, Canada

出版社

Humana Press

出版时间

2018年09月05日

装帧

ＩＳＢＮ

9781493985043

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页码

355

语种

英文

版次

Softcover Repri

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This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques.

Cutting-edge and comprehensive, Pre-Eclampsia: Methods and Protocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship.

Diagnostic Imaging: Ultrasound.-燘iomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the Cobas e 411 Analyzer.-燗ssessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia.-燫ole of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia.-燝enetic Approaches in Pre-Eclampsia.-燛pigenetics and Pre-Eclampsia: Programming of Future Outcomes.-營nflammatory and Immune System Markers.-燤ethods to Enrich Exosomes from Conditioned Media and Biological Fluids.-營solation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants.-燨ptimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation.-燩roteomics Method to Identification of Protein Profiles in Exosomes.-燞arvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental Explants.-燯se of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index.-燛x Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects.-營mmunohistological Techniques.-燯sing a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin.-營solation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta.-燗nalyzing Trophoblast Function Using Cell-Based Assays.-營solation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis.-燗n Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro.-營n Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases.-燤easurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System.-燙o-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis.-燩lacental Lipid Transport.-燛G-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal Model.-燫eal-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-Eclampsia.-燩hase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-Eclampsia.-燗 Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP Trial.

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