图书简介
This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Group A Streptococcus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Detection of Streptococcus pyogenes Virulence Factors.-Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens.-The emm-cluster Typing System.-Protocols for Tn-seq Analyses in The Group A Streptococcus.-Genetic Manipulation of Group A Streptococcus ? Gene Deletion by Allelic Replacement.-Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging.-Whole Genome Sequence Analysis and Population Genomics of Group A streptococci.-Group A Streptococcus Transcriptome Analysis.-Protocol for Proteome Analysis of Group A Streptococcus.-Investigation of GroupA Streptococcal Interactions with Host Glycan Structures using High Throughput Techniques: Glycan Microarray Analysis using Recombinant Protein and Whole Cells.-Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins.-Expression and Purification of Collagen-like Proteins of Group A Streptococcus.-Detection of Fibronectin-binding Proteins of Streptococcus pyogenes using Ligand Blot Analysis.-Morphology and Ultrastructure of Group A Streptococcus Biofilms.-Dynamic Interactions of Group A Streptococcus with Host Macrophages.-Identification of Group A Streptococcus-containingautophagosome-likevacuoles.-Flow Cytometry-based Assays to Quantify Complement Deposition and Neutrophil Uptake of Group a Streptococcus.-Live Cell Microscopy and Flow Cytometry to study Streptolysin S Mediated Erythrocyte Hemolysis.-Isolation of Monoclonal Antibodies to Group A Streptococcus Antigens Using Phage Display.-Assays to Analyse Adhesion of Group A Streptococcus to Host Cells.-The use of Galleria mellonella (wax moth) as an Infection Model for Group A Streptococcus.-A Superficial Skin Scarification Method in Mice to Mimic Streptococcus pyogenes Skin Infection in Humans.-A Mouse Nasopharyngeal Colonisation Model for Group A Streptococcus.-Humanised Plasminogen Mouse Model to Study Group A Streptococcus Invasive Disease.-Lacefield Whole Blood Killing Assay to Evaluate Vaccine Efficacy.-An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera.-Validation of Suitable Carrier Molecules and Target Genes for antisense Therapy using Peptide-coupled Peptide Nucleic Acids (PNAs) in Streptococci.-Interfering with the Folding of Group A Streptococcal pili Proteins.-Use of Streptolysin O (SLO) to Study the Function of Lipid Rafts.-Engineering of Group A Streptococcus Isopeptide Bonds into Immunoglobulin-like Protein Domains.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐