Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

图书简介

Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

Basic Principles of Fluorescence and Energy Transfer.- Applications.- Multiplex Detection of Mutations.- Detection of Salmonella Species in Foodstuffs.- Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR.- Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma.- Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR.- High-Throughput Real-Time PCR.- Design and Use of Scorpions Fluorescent Signaling Molecules.- Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification.- New Technologies.- EasyBeacons? for the Detection of Methylation Status of Single CpG Duplets.- Quenched Autoligation Probes.- HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination.- FIT-Probes in Real-Time PCR.- SNP Genotyping by Unlabeled Probe Melting Analysis.- Non-Nucleic Acid Targets.- Molecular Beacons for Protein?DNA Interaction Studies.- DNA Polymerase Profiling.- Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions.- Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols（Methods in Molecular Biology） 医学遗传学

Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols（Methods in Molecular Biology）
医学遗传学