RNA Nanostructures:Methods and Protocols

图书简介

This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field.

A New Method to Predict Ion Effects in RNA Folding.- Computational Generation of RNA Nanorings.- Protocols for Molecular Dynamics Simulations of RNA Nanostructures.- Rolling Circle Transcription for the Self-Assembly of Multimeric RNAi Structures and its Applications in Nanomedicine.- Computational Prediction of the Immunomodulatory Potential of RNA Sequences.- Co-Transcriptional Production of Chemically Modified RNA Nanoparticles.- Supported Fluid Lipid Bilayer as a Scaffold to Direct Assembly of RNA Nanostructures.- Evaluation of Thermal Stability of RNA Nanoparticles by Temperature Gradient Gel Electrophoresis (TGGE) in Native Condition.- Design and Crystallography of Self-Assembling RNA Nanostructures.- X-Aptamer Selection and Validation.- Design and Preparation of Aptamer-siRNA Chimeras (AsiCs) for Targeted Cancer Therapy.- Cellular Delivery of siRNAs using Bolaamphiphiles.- Preparation and Optimization of Lipid-Like Nanoparticles for mRNA Delivery.- Chitosan Nanoparticles for miRNA Delivery.- Synthesis of PLGA/Lipid Hybrid Nanoparticles for siRNA Delivery using the Emulsion Method PLGA-PEG/Lipid Nanoparticles for siRNA Delivery.- Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA.- Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection.- Intracellular Re-Association of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.- Construction and In Vivo Testing of Prokaryotic Riboregulators.- Preparation of a Conditional RNA Switch.- Rational Engineering of a Modular Group I Ribozyme to Control its Activity by Self-Dimerization.- CRISPR-Cas RNA Scaffolds for Transcriptional Programming in Yeast.- Using Planar Phi29 pRNA Three-Way Junction to Control Size and Shape of RNA Nanoparticles for Biodistribution Profiling in Mice.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

RNA Nanostructures:Methods and Protocols（Methods in Molecular Biology） 医学遗传学

RNA Nanostructures:Methods and Protocols（Methods in Molecular Biology）
医学遗传学