图书简介
This volume discusses methods for the study of multipotent and pluripotent
stem cells of the hair follicle. The stem cells described are involved in both
the growth of the hair follicle and its production of the hair shaft, as well
as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of
the Hair Follicle: Methods and Protocols also explores very unexpected
results such as that of the hair follicle-associated-pluripotent (HAP) stem
cells, which not only have the capability for regenerating the hair follicle
sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell
types not associated with the hair follicle—these include glial cells, motor
neurons, and beating cardiac muscle cells. The potential for HAP stem cells for
regenerative medicine is also discussed in detail. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Cutting-edge
and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and
Protocols is a valuable resource for researchers interested in this
field.
Introduction to Hair-Follicle-Associated
Pluripotent (HAP) Stem Cells.- Nestin-Based Reporter Transgenic Mouse Lines.- Discovery of HAP Stem Cells.- Peripheral-Nerve and Spinal-Cord Regeneration in
Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells.- Construction
of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle
Neural Crest Stem Cells.- Nestin-Expressing
Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term
3D Gelfoam Histoculture.- Isolation and Culture of Neural Crest Stem
Cells from Human Hair Follicles.- Isolation of Mouse Hair Follicle Bulge Stem Cells
and their Functional Analysis in a Reconstitution
Assay.- Hair Follicle Regeneration by Transplantation
of a Bioengineered Hair Follicle Germ.- Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation.- Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.- Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.- Isolation and Fluorescence Activated Cell-Sorting of
Mouse Keratinocytes Expressing ?-Galactosidase.- Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord
of Mice.- Long-Term Gelfoam® Histoculture of
Hair-Shaft-Producing Mouse Whisker Follicles.- From Hair to Heart: Hair Follicle-Associated-Pluripotent
(HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells.- Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium.- Cryopreservation of Intact Hair Follicle Maintains Pluripotency
of Nestin-Expressing (HAP) Stem Cells.
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