Proteomics:Methods and Protocols

Proteomics:Methods and Protocols（Methods in Molecular Biology）
生物数学

原价：

￥ 2016.25

售价：

优惠

平台大促低至8折优惠

发货周期：外国库房发货，通常付款后3-5周到货

作者

Jörg Reinders, University of Regensburg Institute of Functional Genomics, Regensburg, Germany; Albert Sickmann, Institut für Spektrochemie und Angewandte Spektroskopie (ISAS), Dortmund, Germany

出版社

Humana Press

出版时间

2009年06月29日

装帧

精装

ＩＳＢＮ

9781607611561

复制

页码

441

开本

语种

英文

综合评分

暂无评分

图书详情
目次
买家须知
书评（0）
权威书评（0）

图书简介

Since the development of the concept of “proteomics”, great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.

to Proteomics.- Electrophorestic Separations.- High-Resolution Two-Dimensional Electrophoresis.- Non-classical 2-D Electrophoresis.- Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis.- Mass Spectrometry and Tandem Mass Spectrometry Applications.- MALDI MS.- Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery.- A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides.- Multidimensional Protein Identification Technology.- Characterization of Platelet Proteins Using Peptide Centric Proteomics.- Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis.- Quantitative Proteomics.- Liquid Chromatography–Mass Spectrometry-Based Quantitative Proteomics.- iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification.- Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis.- Interpretation of Mass Spectrometry Data.- Algorithms and Databases.- Shotgun Protein Identification and Quantification by Mass Spectrometry.- Analysis of Protein Modifications.- Proteomics Identification of Oxidatively Modified Proteins in Bra.- Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry.- Subcellular Proteomics.- VII Subcellular Proteomics Organelle Proteomics: Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins.- Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems.- Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry.- Analysis of Protein Interactions.- Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag.- Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli.- Bioinformatical Approaches to Detect and Analyze Protein Interactions.

Trade Policy 买家须知