图书简介
Molecular biology has revolutionized research into vascular disease. Over the past 20 years molecular techniques have enabled us to both elucidate - lecular mechanisms in vascular disease and identify appropriate therapies. The vast explosion in technical knowledge and the array of protocols that become more advanced and intricate by the day lead us into new and exciting areas of research that were previously unobtainable. Vascular Disease: Molecular Biology and Gene Transfer Protocols - scribes today?s most powerful molecular methods for the investigation of the pathogenesis of vascular disease. The protocols are highly detailed, allowing beginners who have little experience in either vascular biology or molecular biology to embark on new molecular projects. This book is also suited to more experienced molecular biologists who wish to grasp new methods for stu- ing the involvement of genes in normal vascular physiology and in diseased states. It is well established that cardiovascular disease progression has a s- stantial genetic influence. Part I describes three methods that have been used successfully to identify specific mutations in candidate genes involved in c- diovascular disorders. These mutations include both single-stranded conf- mational polymorphism analysis and heteroduplex detection methods. In addition, technology to map new genes to specific regions of chromosomes by high-resolution mapping is described.
Genetics in Vascular Disease.- Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis.- Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis.- Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes.- Isolation of Genes Expressed in Vasular Tissue.- Efficient Extraction of RNA from Vascular Tissue.- Preparation of cDNA Libraries from Vascular Cells.- Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells.- Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR.- Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques.- The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells.- Identification of Novel Protein Kinases in Vascular Cells.- Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells.- Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes.- Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells.- Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells.- Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells.- Molecular Analysis of MRNA Expression in Vascular Cells.- Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis.- Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases.- Localization of Gene Expression in Human Atherosclerotic Lesions by In SituHybridization.- RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue.- Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissus.- Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL).- In Vitro Detection of Apoptosis in Isolated Vascular Cells.- Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis.- In Vitro Methods to Express Foreign Genes in Vasular Cells.- Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells.- Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells.- Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells.- High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection.- Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Marker.- Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells.- Embryonal Stem (ES) Cell-Derived Macrophages.- Delivery of Recombinant Adenoviruses to Human Saphenous Vein.- In Vivo Vascular Gene Transfer Protocols.- An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization.- Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In Vivo.- Gene Delivery to Rabbit Arteries Using the Collar Model.- Delivery of Antisense Oligonucleotides to the Vascular Wall.- Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo.
Trade Policy 买家须知
- 关于产品：
- ● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
- ● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
- ● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：- 由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
- 签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
- ● 缺页/错页/错印/脱线
关于发货时间：- 一般情况下：
- ●【现货】 下单后48小时内由北京（库房）发出快递。
- ●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
- ● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
- ● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：- 由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间：- 010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
- 其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：- ● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐
