图书简介
This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics. Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.
Historical Note: Lessons learned from developing the first semi-defined medium for culture of one-cell rabbits embryos to expanding blastocysts.- Culture of the mouse preimplantation embryo.- In-vitro culture of rat pre-implantation embryos.- Enabling hamster embryo culture system: development of preimplantation embryos.- Culture of preimplantation rabbit embryos.- In vitro maturation, fertilization, and culture of pig oocytes and embryos.- In vitro culture of late stage pig embryos in a chemically defined medium, Porcine Blastocyst Medium (PBM).- Production and culture of the bovine embryo.- In vitro embryo production in sheep.- In vitro culture of embryos from LOPU-derived goat oocytes.- Small ruminants: prepubertal oocyte donor.- In vitro production of bison embryos.- In vitro embryo production in buffalo species (Bubalis bubalis).- In vitro culture of deer embryos.- In vitro culture of camelid embryos.- In vitro culture of embryos from horses.- In vitro culture of embryos from domestic cats.- The domestic dog embryo: in vitro fertilization, culture, and transfer.- Culture of mink preimplantation embryos.- Culture of marsupial oocytes and conceptuses.- In vitro culture of embryos from the common marmoset (Callithrix jacchus).- In vitro culture of embryos from the cynomolgus macaque (Macca fascicularis).- In vitro culture of rhesus macaque (Macaca mulatta) embryos.- Culture of human preimplantation embryos in a clinical ART setting.- Isolation and culture of peri-implantation and early post-implantation mouse embryos.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐