Microbial Transposon Mutagenesis:Protocols and Applications(Methods in Molecular Biology)

微生物转座子诱变:协议与应用

分子生物学

原   价:
1989.00
售   价:
1591.00
优惠
平台大促 低至8折优惠
发货周期:预计8-10周发货
出  版 社
出版时间
2019年08月04日
装      帧
精装
ISBN
9781493995691
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页      码
200
语      种
英文
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图书简介
This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.
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