图书简介
Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbookremains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design andorganizationof an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world.With the addition oflearning key points and review questions at the beginning and end of each chapter, this new edition ofIn Vitro Fertilizationis a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicingreproductive endocrinologists, urologists, and embryologists also will find value in the book, as willinfertility researchers.
Journey of Human Gametes In Vitro--1978 to 2018.-Setting up an ART unit - Planning, Design and Construction.-Building the Laboratory.-Air Quality Management.-Organization And Workflow In A Fertility Clinic.-Risk and Safety in the IVF Clinic.-Control of variables.-Daily, Weekly, and Regular Preparations for the IVF Laboratory.-Essential Instruments and Disposable Supplies for an IVF Laboratory.-Co2 and Low-O2 Incubators.-What to consider when selecting a LAF, Class II Cabinet or Isolette for your ART program?.-Culture Media in IVF: Decisions for the Laboratory.-Assessment Of Oocyte Quality.-Oocyte Denuding.-Interrupted or Uninterrupted Culture to the Blastocyst Stage in a Single Medium.-Sequential Media for Human Blastocyst Culture.-In Vitro Maturation of Human Oocytes.-Design and Development of Simplified, Low Cost Technologies for Clinical IVF: Applications in High and Low Resource Settings.-In vitro culture of oocytes and embryos with micro-vibration: “naturalization” of routine IVF procedure.-Microfluidics for Gamete Manipulation and Embryo Culture.-Time-Lapse Microscopy for Embryo Culture and Selection.-Sperm Assessment: Traditional Approaches and Their Indicative Value.-Sperm Assessment: Novel Approaches and Their Indicative Value.-Assessment of Sperm Chromatin Damage by TUNEL Method Using Bench Top Flow Cytometer.-Sperm processing in ART.-PESA/MESA/TESA/TESE Sperm Processing.-Processing Sperm Samples in HIV-Positive Patients.-An Emerging Medical Device: Electrophoretic Sperm Separation.-Magnetic Activated Cell Sorting of Human Spermatozoa.-Intrauterine Insemination.-Conventional IVF Insemination.-Intra-Cytoplasmic Sperm Injection.-ntracytoplasmic Morphologically Selected Sperm Injection.-Intracytoplasmic Morphologically Selected Sperm Injection.-Oocyte Activation Deficiency and Advances to Overcome.-Hydraulic Manipulators for ICSI.-Research Instruments Micromanipulation Systems.-Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.-Oocyte Treatment and Preparation for Microinjection.-Piezo-ICSI.-Traditional Embryo Morphology Evaluation: From the Zygote to the Blastocyst Stage.-Predicting Embryo Implantation Potential Using Video Monitoring By the Embryoscope Time-Lapse System.-Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis.-Proteomics and Metabolomics.-Amino Acid Turnover as a Biomarker of Embryo Viability.-Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology.-Assisted Hatching of Human Embryos for Successful Implantation.-Polar Body Biopsy.-Cleavage-Stage Embryo Biopsy.-Current and Novel Methods for Chromosome Testing.-Embryo Biopsy for PGD: Current Perspective.-Sperm Cryopreservation.-Technologies of Cryoprotectant-free Vitrification of Human Spermatozoa: Asepticity as Criterionn of Effectiveness.-Slow Freezing of Oocytes.-Vitrification: Methods Contributing to Successful Cyopreservation Outcomes.-Oocyte Vitrification and Current Clinical Applications.-Slow Freezing of Embryos.-Vitrification of Embryos.-Ovarian Tissue Cryopreservation.-Establishing and Managing Donor Oocyte Banking.-Single Embryo Transfer.-Embryo Transfer: Techniques and Trouble shooting.-G-CSF and GM-CSF: Clinical applications in reproductive medicine.-The Freeze All Cycle: A New Paradigm Shift in ART.-IVF Data Management: From Clipboards to Smart Apps.-Regulation of the ART Laboratory.-Legislation in the UK.-Regulation, Licensing, and Accreditation of the ART Laboratory in Europe.-Regulation, Licensing, and Accreditation of the ART Laboratory in Brazil.-Regulation, Licensing and Accreditation of ART Laboratories in Australia.-Trouble Shooting In Ivf Laboratories.-Benchmarking and Decision Making in the IVF Laboratory.-Automation, Artificial Intelligence and innovations in the future of IVF.-Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease.-Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.-Embryo culture and phenotype of the offspring.-Traceability in ART.-The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.-Cytoskeletal architecture of human oocytes with a focus on centrosomes and their significant role in fertilization.-Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.-Livestock Production via Micromanipulation.
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