图书简介
This book provides both historical perspective and practical information to support researchers either currently involved in genomic research on rats or planning to begin such a project. In numerous chapters, a detailed protocol is provided for researchers looking to move into a new area of investigation or to leverage a new technology. In other cases, a detailed review of existing models or a description of available resources can help the researcher find, understand, and utilize the information, the data, and the tools that they need to support their research efforts. Written as part of the highly successful Methods in Molecular Biology series, this collection includes the kind of hands-on detail necessary for success in the lab.Authoritative and up-to-date, Rat Genomics explores the rat as a biomedical model uniquely poised to provide the ideal combination of established experimental models, extensive physiological data, and genomic manipulability to facilitate exploration of the underlying biology.
The Rat: A Model Used in Biomedical Research.- Rat Genome Assemblies, Annotation, and Variant Repository.- Rat Genome Databases, Repositories, and Tools.- Next Generation Transgenic Rat Model Production.- Embryonic Stem Cells and Gene Manipulation in Rat.- Protocols for Cryopreservation and Rederivation of Rat Gametes.- Fluorescent Imaging and Microscopy for Dynamic Processes in Rats.- Library Preparation for Multiplexed Reduced Representation Bisulfite Sequencing with a Universal Adapter.- Characterization of the Rat Gut Microbiota via 16S rRNA Amplicon Library Sequencing.- Networking in Biology: The Hybrid Rat Diversity Panel.- Using Heterogeneous Stocks for Fine-Mapping Genetically Complex Traits.- Mapping Mammary Tumor Traits in the Rat.- Rat Models of Metabolic Syndrome.- Genomic Research in Rat Models of Kidney Disease.- Rat Models of Exercise for the Study of Complex Disease.- Behavioral Genetic Studies in Rats.
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