Cereal Genomics:Methods and Protocols

图书简介

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping,next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Cereal Genomics: Methods and Protocolsto ensure successful results in the further study of this vital field.

A Low-Cost High-throughput Method for Plant Genomic DNA Isolation.-Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement.-Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping.-Sequencing and Assembling Genomes and Chromosomes of Cereal Crops.-Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing.-Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR.-DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics.-Genome-wide Identification of Regulatory DNA Elements in Crop Plants.-Genome-wide Profiling of Histone Modifications with ChIP-seq.-Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.-Genome-wide Identification of Allele-Specific Gene Expression in a Parent?of?Origin Specific Manner.-Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP).-Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis.-Emerging Genome Engineering Tools in Crop Research and Breeding.-CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids.-Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne.-Agrobacterium Transformation in the Rice Genome.-In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays.

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Cereal Genomics:Methods and Protocols（Methods in Molecular Biology） 谷物基因组学：方法与协议 农艺学

Cereal Genomics:Methods and Protocols（Methods in Molecular Biology）

谷物基因组学：方法与协议
农艺学