图书简介

Assessing and Improving Protein Sample Quality.-A Familiar Protein-Ligand Interaction Revisited with Multiple Methods.-Interactions of a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow Kinetics.-Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins.-Isothermal Titration Calorimetry.-Measuring the Kd of Protein-Ligand Interactions Using Microscale Thermophoresis.-Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Preparing Functionalized Lipid Layers for the Study of Complex Protein-Ligand Interactions.-Indirect Detection of Ligand Binding by Thermal Melt Analysis.-The Use of Acoustic Mist Ionization Mass Spectrometry for High-throughput Screening.-Ligand Discovery – High Through-put Binding: Fluorescence Polarization (Anisotropy).-Fragment Screening by NMR.-A Quick Primer in Fluorescence-based Equilibrium and Pre-Steady State Methods for Determining Protein-Nucleotide Affinities.-Measurement of Nucleotide Hydrolysis using Fluorescent Biosensors for Phosphate.-Gel-based Analysis of Protein-Nucleic Acid Interactions.-Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome using SwitchSENSE.-Biolayer Interferometry: Protein-RNA Interactions.-Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip.-Characterization of Protein Nucleic Acid complexes by Size-Exclusion Chromatography Coupled with Light Scattering, Absorbance and Refractive Index Detectors.-Analytical Ultracentrifugation for Analysis of Protein Nucleic Acid Interactions.-Studying RNA-Protein Complexes Using X-ray Crystallography.-Flow Linear Dichroism of Protein-membrane Systems.-Probing Protein-Membrane Interactions and Dynamics Using Hydrogen-Deuterium eXchange Mass Spectrometry (HDX-MS).

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