Helicobacter Pylori

图书简介

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader throughoverviews onH. pyloriinfection, protocols for analyzing human clinical samples, and protocols for characterizingH. pylori, its functional components and its interactions with host cells.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Helicobacter pylori: Methods and Protocolsaims to be auseful practical guide to researchers to help further their study in this field.

An Overview of Helicobacter pylori Infection.-The Urea Breath Test for the Non-Invasive Detection of Helicobacter pylori.-Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice Analysis.-Molecular Detection of Antibiotic-resistant Helicobacter pylori.-Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test.-Culture-based Antimicrobial Susceptibility Testing for Helicobacter pylori.-Purification of Total RNA from Stomach Tissue Biopsies.-Gene Expression Analysis by Reverse TranscriptionQuantitativePCR.-A Method to Investigate the Helicobacter pylori-associated DNA Methylome.-Profiling the Gastrointestinal Microbiota.-Helicobacter pylori Virulence Factor Genotyping.-Generation of Isogenic Mutant Strains of Helicobacter pylori.-Isolation of Outer Membrane Vesicles from Helicobacter pylori.-A Mouse Model of Helicobacter pylori Infection.-Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa.-Evaluating Host Responses to Helicobacter pylori using ELISA and Western Blot.-Analysis of Innate Immune Responses to Helicobacter pylori.-Analysis of T Cell Responses to Helicobacter pylori Infection.-Miniaturised In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-resistance.

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Helicobacter Pylori（Methods in Molecular Biology） 幽门螺杆菌 内科学

Helicobacter Pylori（Methods in Molecular Biology）

幽门螺杆菌
内科学