The field of biophysical mass spectrometry continues to grow. One of the most important developments witnessed since the publication of the first edition of the book is the gradual acceptance of these tools within the biopharmaceutical industry and regulatory agencies as reliable methods to study architecture and dynamics of biomolecules. This book thoroughly summarizes the variety of MS techniques and strategies related to biomolecular architecture, dynamics, and interactions; it also addresses a wide variety of biophysical problems in biomolecular dynamics and higher order structure. The text presents a discussion of general biophysical concepts and a brief overview of traditional biophysical techniques before going into the more advanced concepts of mass spectrometry. The main aspect of the book is its coverage of MS-based methods for studying higher order structures and biopolymers. The combination of theoretical background and technical aspects of recent developments gives the reader a clear sense of current capabilities and an understanding of the future of experimental MS techniques. The second edition gives an up-to-date and expanded coverage of experimental methodologies and addresses the specific needs of the new and rapidly growing segment of the practitioners of this technique in the biopharmaceutical industry. The updated and significantly expanded presentation of MS techniques used in biophysics and structural biology are preceded by a discussion of general biophysical concepts related to biopolymer structure, dynamics and interaction, a brief overview of traditional biophysical techniques, and an in-depth discussion of modern mass spectrometric hardware used in experimental studies of biomolecular structure and dynamics. Also new is the coverage of Other Biopolymers and Synthetic Polymers of Biological Interest such as Nucleic Acids (DNA and RNA) and Oligosaccharides.

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