How Enzymes Work:From Structure to Function

图书简介

This book starts with an introduction to various enzymes to show how interesting enzymes are. It is followed by historical kinetic studies on enzymes and the overall rapid-reaction kinetics. The subsequent topics describe the basics of protein structure, the control of enzyme activity, and the purification of enzymes. A case on the kinetic and structural studies of L-phenylalanine oxidase is also presented. There are many good books on enzyme kinetics, but only a few books describe their kinetic and structural aspects. This book deals with both and contains many references that can be good sources for further readings. The book is handy and is especially helpful for beginners.

Preface. Chapter One. Introduction. General properties of enzyme. Examples of enzyme. References. Chapter Two. Overall reaction kinetics. Road to the steady state kinetics. Demonstration of the enzyme-substrate complex. Meaning of steady state. Kinetic parameters. Problems. References. Chapter Three. Factors affecting on the enzyme activity. Enzyme. Substrate concentration. Inhibitor. Problems. References. Chapter Four. Effect of pH, temperature, and high pressure on the enzymatic activity. Effect of pH. Thermodynamics in the enzymatic reaction. Temperature dependence of the enzymatic reaction. Effect of pressure. Effect of temperature and pressure on ?-chymotrypsin-catalyzed reaction. Problems. References. Chapter Five. Measurements of individual rate constants. Rapid mixing techniques. Analysis of the first-order reaction. Problems. References. Chapter Six. Structure of proteins. Amino Acids. Polypeptide and protein. Analysis of primary structure. Three dimensional structure. Tertiary and quaternary structures. Structural motif and loop. Problems. References. Chapter Seven. Active site structure. Active site and active center. Cofactor, coenzyme, prosthetic group. Search of active site. Examples of active site studies. Problems. References. Control of enzyme activity. Regulation by non-covalent interaction. Regulation by covalent Modification. Problems. References. Chapter Nine. Preparation of enzyme. Extraction of enzyme. Purification of enzyme. Purity analysis of enzyme. Problems. References. Chapter Ten. A case study: L-Phenylalanine ocidase (deaminating and decarboxylating). Introduction. Preparation of PAO. Catalytic properties of PAO. Molecular Properties of PAO. Physiological role of PAO. Problems. References. Appendix. Derivation of the rate equation by King-Altman?s method. Physical constants. Conversion of units. Greek numbers and alphabet. Useful softwares and data banks. Genetic code. Solutions for problems. Index.

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How Enzymes Work:From Structure to Function 酶学导论 生物化学

How Enzymes Work:From Structure to Function

酶学导论
生物化学