Phage Engineering and Analysis

图书简介

This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understanding of this important and evolving field.

Protocols for Building and Producing High Diversity Peptide Phage Display Libraries.- Generation of a Naïve Human scFv Phage Display Library and Panning Selection.- Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance.- Combining a Base Deaminase Mutator with Phage-Assisted Evolution.- Understanding the Structural Requirements of Peptide-Protein Interaction and Applications for Peptidomimetic Development.- Contracting the Host Range of Bacteriophage T7 Using a Continuous Evolution System.- Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip.- Targeted Genome Editing of Virulent Pseudomonas Phages using CRISPR-Cas3.- Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods.- Liquid Glycan Array.- Electron Microscopy Methods for Phage-Based Study.- Visualization of Engineered M13 Phages Bound to Bacterial Targets by Transmission Electron Microscopy.- Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination using Cryo-Electron Microscopy.- Obtaining Detailed Phage Transcriptomes using ONT-Cappable-Seq.- Multi-Disciplinary Methods for Screening Toxic Proteins from Phages and Their Potential Molecular Targets.- CRISPRpi: Inducing and Curing Prophage using CRISPR-Interference.- Isolation of Bacteriophages on Actinobacteria Hosts.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Phage Engineering and Analysis（Methods in Molecular Biology） 噬菌体工程与分析：方法与协议 生物工程

Phage Engineering and Analysis（Methods in Molecular Biology）

噬菌体工程与分析：方法与协议
生物工程