图书简介
This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, the detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and in silicon analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signalling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in the proposed book.In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin.Consequently, the proposed manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research.
Section 1. Isolation and cultivation methods for Cyanobacteria.- Chapter 1. Collection of toxic Cyanobacteria from fish sample.- Chapter 2. Identification of toxic blooms of cyanobacteria in fresh water (lake, ponds) habitat.- Chapter 3. Identification of toxic blooms of cyanobacteria in marine water habitat.- Chapter 4. Identification of toxic blooms of cyanobacteria in Mangrove forest.- Chapter 5. Identification of toxic blooms of cyanobacteria in Estuarine habitat.- Chapter 6. Identification of toxic blooms of cyanobacteria in Saltpan habitat.- Chapter 7. Sampling and Identification of toxic cyanobacteria in Landfill Leachate.- Chapter 8. Handling and processing of Cyanobacterial toxin containing pond and lake water samples.- Chapter 9. Isolation of toxin producing Cyanobacteria from aquatic samples with Anabaena.- Chapter 10. Isolation of toxin producing Cyanobacteria from aquatic samples- Anabaenopsis sp.- Chapter 11. Isolation of toxin producing Cyanobacteria from aquatic samples with Cylindrospermopsis sp.- Chapter 12. Isolation of toxin producing Cyanobacteria from aquatic sample with Nodularia sp.- Chapter 13. Isolation of toxin producing Cyanobacteria from aquatic samples with Nostoc sp.- Chapter 14. Isolation of toxin producing Cyanobacteria from aquatic samples with Schizotrix.- Chapter 15. Isolation of toxin producing Cyanobacteria from aquatic samples with Lyngbya.- Chapter 16. Isolation of toxin producing Cyanobacteria from aquatic samples with Raphidiopsis.- Chapter 17. Isolation of toxin producing Cyanobacteria from aquatic samples – Oscillatoria.- Chapter 18. Isolation of toxin producing Cyanobacteria from aquatic samples – Planktothrix.- Chapter 19. Analysis of Cyanobacterial species composition and variation analysis among toxic blooms.- Chapter 20. Confocal Laser Scanning Microscope (CLSM) Identification of Toxic Cyanobacteria from Algal Bloom.- Chapter 21. Determination of Cyanotoxin patterns in strains and species of cyanobacteria.- Chapter 22. Chemical and toxicological studies of the toxic freshwater cyanobacterium - Microcystis aeruginosa.- Chapter 23. Chemical and toxicological studies of the toxic freshwater cyanobacterium - Anabaena flosaquae.- Chapter 24. Risk assessment of microcystin in the water resources.- Chapter 25. Extraction and quantification of BMAA from water sample.- Chapter 26. Extraction and quantification of BMAA from fish tissue.- Chapter 27. Postmortem sampling of Cyanobacteria in the case of suspected Drowning.- Section II. Toxicity analysis of Cyanotoxin.- Chapter 28. Detection of Cyanotoxins using Vertebrate bioassays in Mice.- Chapter 29. Detection of Cyanotoxin toxicity in Fish.- Chapter 30. Detection of Cyanotoxins using Invertebrate bioassays in brine Shrimp.- Chapter 31. Identification of Cyanobacterial toxins from Lichen thalli.- Chapter 32. Detection of Cyanotoxins using Bacterial bioassays.- Chapter 33. Detection of Cyanotoxins – Bioassay using plants.- Chapter 34. In vitro assay for determining cyanotoxin using serological methods.- Chapter 35. In vitro assay for determining cyanotoxin using cell line method- Hepatotoxicity (cell lines- HepG2, CaCo2, and V79).- Chapter 36. In vitro assay for determining cyanotoxin using cell line method- Neurotoxicity ( Neuro-2A neuroblastoma cell).- Chapter 37. Genotoxicity of Cyanotoxins.- Chapter 38. In silico analysis of cyanotoxin using computational tools.- Section III. Cyanobacterial toxin Extraction, detection and quantification.- Chapter 39. Extraction of Cyanotoxins using filtration and other methods.- Chapter 40. Extraction of Cyanotoxin by filtration and other methods from Biological materials (animal tissue: fish and mussels).- Chapter 41. Extraction and quantification of Nodularins from fish samples by LC-MS/MS.- Chapter 42. Extraction and quantification of Nodularins
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