图书简介
This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.
In vivo cross-linking and co-immunoprecipitation procedure to analyze nuclear tRNA export complexes in yeast cells.- Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in situ Hybridization in Whole Mount Drosophila Testes.- Electrophoretic mobility shift assay (EMSA) and microscale thermophoresis (MST) methods to measure interactions between tRNAs and their modifying enzymes.- Mapping of RNase P ribozyme regions in proximity with a human RNase P subunit protein using Fe(II)-EDTA cleavage and nuclease footprint analyses.- SHAPE to probe RNA structure and RNA-protein interactions in vitro.- Chemical probing of RNA structure in vivo using SHAPE-MaP and DMS-MaP.- Analysis of RNA-protein interaction networks using RNP-MaP.- Native RNA immunoprecipitation (RIP) for precise detection and quantification of protein-interacting RNA.- In vivo cross-linking and co-immunoprecipitation procedure to analyze nuclear tRNA export complexes in yeast cells.- Identify microRNA targets using AGO2-CLASH (Cross-linking, Ligation, And Sequencing of Hybrids) and AGO2-CLIP (Cross-Linking and Immuno-Precipitation) in cells with or without the microRNA of interest depleted.- Ribosomal Profiling by Gradient Fractionation of Cell Lysates.- Global assessment of protein translation in mammalian cells using polysome fractionation.- Fluorescent in situ detection of RNA-Protein interactions in intact cells by RNA-PLA.- Arresting Spliceosome Intermediates at Various Stages of the Splicing Pathway.- Streamlined purification of RNA-protein complexes using UV crosslinking and RNA antisense purification.- MS2-MBP based affinity purification of nucleus- or cytoplasm-localized lncRNA-protein complexes formed in vivo.- RNA and protein interactomes of an RNA-binding protein tagged with FLAG epitopes using combinatory approaches of genome engineering and stable transfection.- Detecting R-loop formation using a plasmid-based in vitro transcription assayMethods to Study RNA-Chromatin Interactions.- Challenges for Studying and Isolating Extracellular Vesicles from Cell-conditioned Media.- Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.
Trade Policy 买家须知
- 关于产品：
- ● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
- ● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
- ● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：- 由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
- 签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
- ● 缺页/错页/错印/脱线
关于发货时间：- 一般情况下：
- ●【现货】 下单后48小时内由北京（库房）发出快递。
- ●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
- ● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
- ● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：- 由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间：- 010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
- 其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：- ● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐
