图书简介
This volume aims to help new investigators to the field by providing them with the protocols needed to set up clinically relevant animal models of fungal infection, as well as methods for in vivo immunology.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Antifungal Immunity: Methods and Protocols aims to be comprehensive guide for researchers in the field
Quantifying the mechanical properties of yeast Candida albicans using atomic force microscopy-based force spectroscopy.- Standardization of Galleria mellonella as an infection model for Malassezia furfur and Malassezia pachydermatis.- Mouse organotypic brain slice cultures: a novel model for studying neuroimmune responses to cryptococcal brain infections.- Zebrafish larvae as an experimental model of cryptococcal meningitis.- Immunological Analysis of Cryptococcal Meningoencephalitis in a Murine Model.- Mouse Model of Latent Cryptococcal Infection and Reactivation.- Adoptive transfer of Cryptococcus neoformans-specific CD4 T-cells to study anti-fungal lymphocyte responses in vivo.- Meningeal whole mounts for imaging CNS fungal infection.- An antibiotic-free model of Candida albicans colonisation of the murine gastrointestinal tract.- An experimental model of chromoblastomycosis caused by Fonsecaea sp species.- Modelling Chronic Coccidioidomycosis in Mice.- Mouse models of phaeohyphomycosis.- Genetic mouse models of Pneumocystis pneumonia.- Mouse models of Mucormycosis.- Bioluminescence imaging, a powerful tool to assess fungal burden in live mouse models of infection.- Micro-Computed Tomography to visualize and quantify fungal infection burden and inflammation in the mouse lung over time.
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