Cytochrome P450

Cytochrome P450（Methods in Pharmacology and Toxicology）

细胞色素 P450：体外方法与方案
药理学

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作者

Zhengyin Yan Gary W. Caldwell

出版社

Humana Press

出版时间

2022年07月10日

装帧

平装

ＩＳＢＮ

9781071615447

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页码

392

开本

10.00 x 7.00 x 0.81

语种

英文

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This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.

Cytochrome P450: In Vitro Methods and Protocols.- Characteristics of Major Drug Metabolizing Cytochrome P450 Enzymes.- Quantitative Determination of Cytochrome P450 Using LC/MS/MS.- Spectral Quantitation of Total Cytochrome P450 in Microsomes Using a Single-Beam Spectrophotometer.- Cytochrome P450 Enzyme Kinetics: Km and Vmax Determinations Using Metabolite Formation and Substrate Depletion Methods.- Cytochrome P450 Inhibition Assay Using Human Liver Microsomes.- Evaluation of Time-Dependent Cytochrome P450 Inhibition Using the Area Under the Curve Shift Method.- Assessing Cytochrome P450 Time Dependent Inhibition (IC50 Shift Assay) Using Both Diluted and Non-Diluted Methods.- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Pregnane X Receptor (PXR, NR1I2) Activation.- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Aryl Hydrocarbon Receptor (HLHE76, AhR) Activation and Antagonism.- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Direct and Indirect Activation of Constitutive Androstane Receptor (CAR, NR1I3).- Determination of In Vitro Cytochrome P450 Induction Potential Using Cryopreserved Human Hepatocytes.- Assessing Cytochrome P450 3A (CYP3A) Induction and Suppression Using Primary Human Hepatocyte Spheroids.- Cytochrome P450-Mediated Metabolic Stability Assay in Liver Microsomes.- Assessment of Cytochrome P450 Metabolic Clearance Using Hepatocyte Suspension.- Cytochrome P450 Knock-Out Assay with Non-Selective Inhibitors.- Differentiation of Cytochrome P450-Mediated from Non-CYP-Mediated Metabolism: Aldehyde Oxidase and Xanthine Oxidoreductase.- In Vitro Reaction Phenotyping of Cytochrome P450 Enzymes.- Cytochrome P450-Mediated Drug Bioactivation Assay: An Untargeted High Resolution Accurate Mass LC/MS Assay.- Simultaneously Assessing Concentration Changes in 20 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: An Untargeted Metabolomics GC/MS Assay.- Simultaneously Assessing Concentration Changes in 17 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: A Quasi-Untargeted Metabolomics LC/MS Assay.- Assessing Amino Acid Concentration Changes as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism.

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