Single-Molecule Science

图书简介

Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology.

Preface; I. Super Resolution Microscopy and Molecular Imaging Techniques to Probe Biology: 1. Introduction on Single Molecule Science Krishnarao Appasani and Raghu K. Appasani; 2. One molecule, two molecules, red molecules, blue molecules: Methods for quantifying localization microscopy data George Patterson and Gaetan G. Herbomel; 3. Muti-scale fluorescence imaging Ruben Bulkescher, Inn Chung, Jan Philipp Eberle, Holger Erfle, Manuel Gunkel, Jürgen Reymann, Karsten Rippe, Guido Sauter, Ronald Simon and Vytaute Starkuviene; 4. Long-read single-molecule optical maps Assaf Grunwald, Yael Michaeli and Yuval Ebenstein; II. Protein Folding, Structure, Confirmation and Dynamics: 5. Single-molecule mechanics of protein nanomachines Gabriel Žoldák and Katarzyna Tych; 6. Post-translational Protein Translocation Through Membranes at the Single Molecule Level Diego Quiroga-Roger, Hilda M. Alfaro-Valdés and Christian A.M. Wilson Moya; III. Mapping DNA Molecules at Single Molecule Level: 7. Observing Dynamic states of single molecule DNA and Proteins using Atomic Force Microscope Jingqiang Li, Sithara Wijeratne and Ching-Hwa Kiang; 8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification Sourav Mishra, Yoonhee Lee and Joon Won Park; IV. Single Molecule Biology to Study Gene Expression: 9. Single molecule detection in the study of gene expression Vipin Kumar, Simon Leclerc and Yuichi Taniguchi.

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Single-Molecule Science 单分子科学：从超高分辨率显微镜到DNA定位和诊断 原子分子物理学

Single-Molecule Science

单分子科学：从超高分辨率显微镜到DNA定位和诊断
原子分子物理学