Nanotechnology for Nucleic Acid Delivery:Methods and Protocols

图书简介

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Toxicological Aspects for Nanomaterial in Humans.- Lactate Dehydrogenase Assasy for Assessment of Polycation Cytotoxicity.- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determincation for Assessment of Polycation-Mediated Cytotoxicity.- Anti-PEG IgM Production Via a PEGylated Nano-Carrier System for Nucleic Acid Delivery.- Near-Infrared Optical Imaging of Nucleic Acid Nanocarrier In Vivo.- Lipids for Nucleic Acid Delivery: Synthesis and Particle Formantion.- Histidine-Rich Cationic Amphipathic Peptides for Plasmid DNA and siRNA Delivery.- Synthesis of Polyethylenimine Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids.- Synthesis of Bioreducible Polycations with Controlled Topologies.- Lyophilization of Synthetic Gene Carriers.- Surface-and Hydrogel-Mediated Delivert of Nucleic Acid Nanoparticles.- Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids.- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films.- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles.- Magnetic And Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery.- Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery.- Lipopeptide Delivery of siRNA to the Central Nervous System.- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.- Liver-Targeted Gene Delivery Through Retrograde Intrabiliary Infusion.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Nanotechnology for Nucleic Acid Delivery:Methods and Protocols（Methods in Molecular Biology） 生物工程

Nanotechnology for Nucleic Acid Delivery:Methods and Protocols（Methods in Molecular Biology）
生物工程