图书简介
Volume 9 of Chemical Mutagens consists mainly of chapters discussing the development and validation of short-term assays to detect the mutagenic effects of environmental chemicals. These chapters include an assay with the grasshopper neuroblast, a comparison of mutagenic responses of human lung-derived and skin-derived diploid fibroblasts, a forward-mutation assay in Salmonella, a multigene sporulation test in Bacillus subtilis, a specific locus assay in mouse lymphoma cells, a study of the induction of bacteriophage lambda, and the granuloma pouch assay. In addition, there are two chapters on the identification of mutagens in cooked food and in human feces. Frederick 1. de Serres Research Triangle Park, North Carolina vii Contents Chapter 1 The Grasshopper Neuroblast Short-Term Assay for Evaluating the Effects of Environmental Chemicals on Chromosomes and Cell Kinetics 1 Mary Esther Gaulden, Jan C. Liang, and Martha J. Ferguson 1. Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. Embryo Supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 1. Species. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 2. Origin of Colonies . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 3. Life Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 4. Colony Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . 6 2. 5. Pathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2. 6. Allergy to Grasshoppers . . . . . . . . . . . . . . . . . . . . . . 14 3. Grasshopper Egg, Embryo, and Cells . . . . . . . . . . . . . . . . . 14 3. 1. The Egg Shell and Membranes . . . . . . . . . . . . . . . . . 14 3. 2. Embryonic Development . . . . . . . . . . . . . . . . . . . . . . 17 3. 3. Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 4. Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 4. 1. Exposure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 4. 2. Preparation of Embryos for Cell Analysis . . . . . . . . . 34 4. 3. Analysis of Mutagen Effects. . . . . . . . . . . . . . . . . 40 . . . 5. Response of the Grasshopper Neuroblast to Mutagens . . . . 50 5. 1. Reproducibility of Data . . . . . . . . . . . . . . . . . . . . . . . 50 5. 2. Radiation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 5. 3. Chemical Mutagens . . . . . . . . . . . . . . . . . . . . . . . . . .
1 In Vivo Mutagenicity Testing Using Somatic Cells of Drosophila melanogaster.- 1. Introduction.- 2. Basic Developmental Biology of Drosophila.- 3. The Wing Mosaic System.- 4. The White/White-Coral Eye Mosaic System.- 5. The Unstable White-Zeste Eye Mosaic System.- 6. Exposure Techniques.- 7. Discussion.- 8. Conclusions.- 9. References.- 2 Structural and Metabolic Parameters Governing the Mutagenicity of Polycyclic Aromatic Hydrocarbons.- 1. Introduction.- 2. Activation Pathways of PAHs.- 3. Contribution of Different Activation Pathways to the Mutagenicity of PAHs.- 4. Metabolic Control of Mutagenic Intermediates of PAHs.- 5. Summary and Conclusions.- 6. Addendum: Recent Developments.- 7. References.- 3 Chromosomal Mutations: The Genetic Approach.- 1. Introduction.- 2. The Main Categories of Chromosomal Mutation.- 3. Genetic Detection of Aneuploidy in the Mouse.- 4. Reciprocal Translocations: Detection and Analysis.- 5. Detection and Study of Other Translocations and of Inversions.- 6. Detection and Study of Deletions.- 7. Conclusions.- 8. References.- 4 Cytogenetic Assays for Mitotic Poisons Using Somatic Animal Cells.- 1. Introduction.- 2. Mitotic Poisons and Their Genomic Effects.- 3. Assays with Diploid Mammalian Cells in Vitro.- 4. Assays with Bone Marrow Cells in Vivo.- 5. Assays with Grasshopper Embryos.- 6. Conclusions.- 7. References.- 5 Detection of Aneuploidy in Drosophila.- 1. Introduction.- 2. Issues.- 3. Assay Systems.- 4. Known Positives.- 5. Summary.- 6. References.- 6 Mammalian Cytogenetic and Genetic Tests for Nondisjunction.- 1. Introduction.- 2. Cytogenetic Tests.- 3. Genetic Test Systems with the Mouse.- 4. Summary and Conclusions.- 5. References.- 7 Using Repair-Deficient Chinese Hamster Ovary Cells to Study Mutagenesis.- 1. Introduction.- 2. Genetic and Biochemical Properties of Repair-Deficient Mutants.- 3. Hypersensitivity of Repair Mutants to DNA-Damaging Agents.- 4. Rapid Detection of DNA-Damaging Agents by Differential Cytotoxicity (DC).- 5. Conclusions.- 6. References.- 8 Transplacental Genotoxic Agents: Cytogenetic Methods for Their Detection.- 1. Introduction.- 2. Animal Studies.- 3. Human Studies.- 4. References.- 9 Computer Automation of Metaphase Finding, Sister Chromatid Exchange, and Chromosome Damage Analysis.- 1. Introduction.- 2. Historical Trends in Computer Automation.- 3. Development of a System for Cytogenetic Mutagenicity Testing.- 4. Future Prospects for Full Automation.- 5. References.- 10 Mutagen-Sensitive Mutants and Chemical Mutagenesis in Drosaphila.- 1. Introduction.- 2. Isolation and Localization of Mutants.- 3. Biochemical Characteristics.- 4. Larval Exposure.- 5. Adult Exposure.- 6. Maternal Effects.- 7. Storage Effect.- 8. Conclusions.- 9. References.- 11 Chromosomal Causes for Fertility Reduction in Mammals.- 1. Introduction.- 2. Numerical Chromosome Mutations of the Sex Chromosomes and Related Conditions.- 3. Structural Chromosome Anomalies.- 4. Combinations of Chromosome Mutants within One Carrier.- 5. Viable Unbalanced Progeny Obtained from Translocation Heterozygote Females and Males.- 6. Why is Chromosomal Infertility More Clearly Expressed in the Male Than in the Female? Consideration of X-Autosome Translocations.- 7. References.- 12 Mutagenesis and Plasmids.- 1. Introduction.- 2. Historical Overview of Plasmids.- 3. Survey of Thirty-Three R Plasmids.- 4. Mechanisms of Plasmid-Mediated Effects on Mutagenesis and Survival.- 5. References.- 13 Mutation in Somatic Cells as Determined by Electrophoretic Analysis of Mutagen-Exposed Chinese Hamster Ovary Cells.- 1. Introduction.- 3. Results and Discussion.- 4. Summary.- 5. References.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐