MERS Coronavirus:Methods and Protocols

图书简介

This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Studying Evolutionary Adaptation of MERS-CoV.-Evaluating MERS-CoV Entry Pathways.-Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing.-Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein.-Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV.-Deducing the Crystal Structure of MERS-CoV Helicase.-Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans.-Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR.-Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay.-Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera.-Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA.-Genetically Engineering a Susceptible Mouse Model for MERS-CoV-InducedAcute Respiratory Distress Syndrome.-Development of a Mouse Adapted MERS Coronavirus.-Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.-Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection.-Histopathologic Evaluation and Scoring of Viral Lung Infection.

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MERS Coronavirus:Methods and Protocols（Methods in Molecular Biology） MERS 冠状病毒：方法与协议 人体免疫学

MERS Coronavirus:Methods and Protocols（Methods in Molecular Biology）

MERS 冠状病毒：方法与协议
人体免疫学