图书简介
The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, “omic” approaches. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,T- Helper Cells: Methods and Protocolsaims to be a useful practical guide to researches to help further their study in this field.
Human and Murine T helper Cell Recovery from Organs and Tissues.-Identification and Purification of Human Memory T Helper Cells from Peripheral Blood.-Transcription Factor Evaluation by Flow Cytometry.-Mass Cytometry Analysis of T Helper Cells.-Murine T Helper Cell Differentiation and Plasticity.-Identification of Follicular T Cell Subsets in Murine and Human Tissues.-Analysis of T Cells In Inflamed Non-Lymphoid Tissues.-TCR V? Evaluation by Flow Cytometry.-Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation.-Evaluation of Secreted Cytokines by Multiplex Bead Based Assay (X MAP Technology, Luminex).-Quantifying Antigen-specific T Cells by Assessing their Antigen-Induced Proliferation.-Flow Cytometric Characterization of Human Antigen-Reactive T helper Cells.-Identification of Human Antigen-Specific CD4+ cells with Peptide-MHC Multimer Technologies.-Human T cell Cloning by Limiting Dilution.-Human Germinal Center Reaction: Assessing T Follicular Helper-B cell Interactions In Vitro.-Study of Signal Transduction Pathways by Phospho-Protein Evaluation.-The Study of Protein-DNA Interactions in CD4+ T Cells using ChIPmentation.-mRNA Transfection of T lymphocytes by Electroporation.-Transduction of Human T Cell Subsets with Lentivirus.-Plasmid- or Ribonucleoprotein-mediated CRISPR/Cas Gene Editing in Primary Murine T Cells.-Generation of Sequencing Libraries for Building Immune Cell Methylomes.-Gene Expression Analysis of T Cells by Single Cell RNA-seq.-Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry.-Metabolomics, Lipidomics and Immunometabolism.-Experimental Mouse Models of Asthma and Analysis of CD4+ T Cells.-Murine T Cell Transfer Colitis as a Model for Inflammatory Bowel Disease.-Experimental Autoimmune Encephalomyelitis.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐