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Section 1: Methods for Studying Microorganisms in Space 1. Microbiology of the Built Environment in Spacecraft Used for Human Flight Jiseon Yang, Starla G. Thornhill, Jennifer Barrila, Cheryl A. Nickerson, Charlie M. Ott and Robert J.C. McLean 2. The Study of Microbial Survival in Extraterrestrial Environments Using Low Earth Orbit and Ground-Based Experiments Karen Olsson-Francis, Nisha K. Ramkissoon, Alex B. Price, David J. Slade, Michael Macey and Victoria K. Pearson 3. Persistence of Fungi in Atypical, Closed Environments: Cultivation to Omics Adriana Blachowicz, Kasthuri Venkateswaran and Clay C.C. Wang Section 2: Molecular Methods 4. Molecular Methods for Studying Microorganisms From Atypical Environments Hirak Ranjan Dash and Surajit Das 5. Measuring Microbial Metabolism in Atypical Environments Wendy Stone and Gideon Wolfaardt 6. Rock-Inhabiting Fungi: Survival on and Alteration of Solid Air-Exposed Surfaces Nicole Knabe and Anna A. Gorbushina 7. Taxonomy of Oral Bacteria Samantha J. Byrne, Catherine A. Butler, Eric C. Reynolds and Stuart G. Dashper 8. Aqueous Methods for Extraction/Recovery of Macromolecules From Microorganisms of Atypical Environments: A Focus on Three Phase Partitioning Mohammed Gagaoua 9. Microbial Community Composition and Predicted Functional Attributes of Antarctic Lithobionts Using Targeted Next-Generation Sequencing and of Bioinformatics Tools Hyunmin Koo, Joseph Hakim, Casey Morrow, Dale T. Andersen and Asim K. Bej Section 3: Microscopic Methods 10. Methods for Collection and Characterization of Samples From Icy Environments Manuel Bedrossian, Jody W. Deming and Jay Nadeau 11. Cryo-Electron Microscopy of Extremely Halophilic Microbes Harold Engelhardt and Daniel Bollschweiler Section 4: Organisms 12. Methods to Study Magnetotactic Bacteria and Magnetosomes Lei Yan and Weijia Xing 13. Molecular Methods to Study Vibrio parahaemolyticus and Vibrio vulnificus From Atypical Environments Indrani Karunasagar, Biswajit Maiti and Ballamoole Krishna Kumar

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