图书简介
Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that high school and tertiary institution teachers and students need for a colourful lab course in bacterial synthetic biology based on chromoproteins and designer antisense RNAs.
馆藏图书馆
Harvard Library
Introduction: What is Synthetic Biology, Exactly?; The iGEM Outbreak; A Synthetic Biology Lab Manual; Genes, Chromoproteins and Antisense RNAs: E. Coli DNA: Chromosomes, Plasmids and Copy Number; Coupling of Transcription and Translation in Bacteria; Promoter and Terminator for Transcription; Ribosome Binding Site (RBS); Codon Bias; Chromoproteins; Small Regulatory RNAs (sRNAs); Lab Rooms and Equipment: The Physical Lab Spaces; Equipment; Safety is Priority #1: Fires; Chemicals; Biological Safety and Disposal; Dangerous Equipment; Lab Course Projects: Time Considerations; Resource Considerations; Project Overview; The Lab Notebook; Part 1. Preparation of Chemical Solutions and Agar Plates; Part 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein Gene; Part 3: Rational Engineering of Chromoprotein Expression Level; Part 4. Other Experiments; Protocols: Protocol 1. Preparation of Solutions and Agar Plates; Protocol 2. Overnight Cultures with Antibiotics; Protocol 3. Biobrick 3A Assembly and Gel Analysis; Protocol 4. Agarose Gel Electrophoresis; Protocol 5. Preparation of Competent E. Coli Cells Using CaCl2; Protocol 6. Transformation of CaCl2-Competent E. Coli Cells; Protocol 7. Bacterial Re-Streaking Techniques; Protocol 8. Lysis of E. Coli Cells with Lysozyme; Protocol 9. PCR; Protocol 10. Reverse PCR Mutagenesis; Protocol 11. Colony PCR; Protocol 12. Gibson Assembly; The “Dreaded” Exam: The Lab as Part of a Course; Powerpoint Slide Presentation; Exam; Course Evaluation; The International Genetically Engineered Machines (iGEM) Competition: Uppsala iGEM 2011 — Show Colour with Colour; Uppsala iGEM 2012 — Resistance is Futile; Appendices: Flow Cytometry and Cell Sorting; Lambda Red Recombination; Chromosomal Integration; Electric Competent Cells; Example of Lab Course Schedule; Equipment List; Order List; Table of BioBrick Parts; Glossary; Recommended Readings.
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