The immune system is not bound by a single tissue but is instead bestowed with the challenge of warding off invading pathogens throughout the body. Constant surveillance of the body requires that the immune system be highly mobile and able to purge pathogens from all tissues. Because each tissue presents its own unique architecture and milieu, it is necessary for the immune system to be as malleable as it is dynamic. For example, how the immune system handles a pathogen in the lung can differ significantly from a pathogen encountered in the gut. Understanding immune complexity in diverse tissue environments is a challenge for researchers. However, advances in imaging have greatly improved our ability to probe the immune system. From snap-shots in time to 4D movies, imaging systems have been used to generate stunning visualizations of immune cells in action throughout the body. These visualizations are not only aesthetically pleasing but they have yielded great advances in our understanding of immune function. This volume provides a synopsis of major insights in immunology revealed using imaging approaches. Seeing is truly believing , and this volume was assembled to recognize past accomplishments and to provide visions of what the future holds in store in this exciting field.

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